Table 2. Identification of proteins which show altered expression in steatosis versus control.
Protein spots were analyzed by MALDI TOF peptide mass fingerprinting and MALDI TOF/TOF peptide fragment fingerprinting as described in experimental section. Spot number indicates the number labeling the spots in Figure 1. For each protein, entry name and protein name are annotated as in SwissProt database. The fold change, the estimated MW and pI, the percentage of the sequence coverage observed (SC%), the number of peptides identified per protein (NP) and the Mascot score are also indicated. Only protein spots that were present on every gel and vary with a significance of p<0.05 were considered.
| Spot number | Entry name | Protein Name | Fold change | MW/pI | S.C.(%) | NP | Mascot score |
|---|---|---|---|---|---|---|---|
| 1 | 1433E_HUMAN | 14-3-3 protein epsilon | 3.4 | 44/4. | 30 | 11 | 116 |
| 2 | GRP78_HUMAN | 78 kDa glucose regulated protein precursor | 2.4 | 45/4.5 | 18 | 11 | 159 |
| 3 | GRP78 + CPNS1_HUMAN | 78 kDa glucose regulated protein precursor + Calpain small subunit 1 | 1.7 | 45/4 | 11+15 | 6+4 | 80/94 |
| 4 | ABHEB_HUMAN | Abhydrolase domain containing protein 14 b | 1.6 | 40/5.5 | 27 | 5 | 200 |
| 5 | NUDT5_HUMAN | ADP sugar pyrophosphatase | 1.7 | 48/4 | 38 | 8 | 124 |
| 6 | NUDT5 + K1C16_HUMAN | ADP sugar pyrophosphatase + keratine type 1 cytoeskeletal 16 | 2.8 | 47/4.2 | 38+20 | 8+8 | 160/99 |
| 7 | ALDH2 + AL1B1_HUMAN | Aldheyde dehydrogenase 2 + Aldheyde dehydrogenase X | 0.5 | 60/6 | 28+24 | 12+12 | 112/97 |
| 8 | APOA1 | Apoliprotein A1 precursor | 1.7 | 45/5 | 47 | 22 | 267 |
| 9 | ACTB_HUMAN | Beta actin | 1.7 | 50/4 | 23 | 11 | 110 |
| 10 | CALR_HUMAN | Calreticulin precursor | 2.3 | 52/3.5 | 15 | 6 | 103 |
| 11 | CALR_HUMAN | Calreticulin precursor | 0.5 | 64/3.2 | 66 | 30 | 404 |
| 12 | CALR_HUMAN | Calreticulin precursor | 0.5 | 62/3.2 | 24 | 13 | 187 |
| 13 | CALU_HUMAN | Calumenin precursor | 2.2 | 55/3.2 | 17 | 7 | 155 |
| 14 | ENPL_HUMAN | Endoplasmin precursor | 1.7 | 42/3.5 | 8 | 7 | 114 |
| 15 | ENPL_HUMAN | Endoplasmin precursor | 2.2 | 40/3.6 | 5 | 5 | 109 |
| 16 | ENPL_HUMAN + PDIA1HUMAN | Endoplasmin precursor + Protein disulfide isomerase precursor | 0.4 | 62/3.8 | 19+12 | 20+6 | 134/133 |
| 17 | ENPL_HUMAN | Endoplasmin precursor | 0.4 | 50/3.5 | 16 | 17 | 166 |
| 18 | FRIL_HUMAN | Ferritin Light chain | 2.9 | 35/5 | 17 | 6 | 187 |
| 19 | HPT_HUMAN | Haptoglobin precursor | 2.6 | 25/3.5 | 10 | 6 | 139 |
| 20 | HS90A_HUMAN | Hsp 90 alpha | 1.8 | 60/4.2 | 10 | 14 | 140 |
| 21 | HS90B_HUMAN | Hsp 90 beta | 2.4 | 45/4 | 6 | 5 | 101 |
| 22 | K1C18_HUMAN | Keratina type I; cytoeskeletal 18 | 2.1 | 57/5 | 62 | 33 | 438 |
| 23 | ACSL1_HUMAN | Long chain fatty acid CoA ligase1 | 2 | 40/6 | 9 | 10 | 137 |
| 24 | NADC_HUMAN | Nicotinate nucleotide pyrophorylase | 1.7 | 55/5.5 | 21 | 7 | 147 |
| 25 | PRDX4_HUMAN | Peroxiredoxin 4 | 2.9 | 42/5 | 49 | 13 | 197 |