Table 3. Identification of proteins which show altered expression in NASH versus control.
Protein spots were analyzed by MALDI TOF peptide mass fingerprinting and MALDI TOF/TOF peptide fragment fingerprinting as described in experimental section. Spot number indicates the number labeling the spots in Figure 1. For each protein, entry name and protein name are annotated as in SwissProt database. The fold change, the estimated MW and pI, the percentage of the sequence coverage observed (SC%), the number of peptides identified per protein (NP) and the Mascot score are also indicated. Only protein spots that were present on every gel and vary with a significance of p<0.05 were considered.
| Spot number | Entry name | Protein Name | Fold change | MW/pI | S.C.(%) | M.P. | Mascot score |
|---|---|---|---|---|---|---|---|
| 1 | CH60_HUMAN | 60kDa HSP | 0.6 | 65/4.5 | 41 | 25 | 250 |
| 2 | GRP78_HUMAN | 78 kDA glucose regulated protein precursor | 0.4 | 70/4.2 | 9 | 6 | 97 |
| 3 | GRP78_HUMAN | 78 kDA glucose regulated protein precursor | 0.5 | 70/4.2 | 39 | 28 | 279 |
| 4 | GRP78_HUMAN | 78 kDA glucose regulated protein precursor | 0.6 | 70/4.2 | 53 | 35 | 358 |
| 5 | A1AG1_HUMAN | Alpha1-acid glycoprotein precursor | 1.8 | 55/5 | 18 | 4 | 90 |
| 6 | CPNS1_HUMAN | Calpain small subunit 1 | 2.5 | 45/4 | 18 | 6 | 130 |
| 7 | CARL_HUMAN | Calreticulin precursor | 2.3 | 65/3.5 | 27 | 17 | 264 |
| 8 | CALU_HUMAN | Calumenin precursor | 2.6 | 67/3.5 | 17 | 7 | 155 |
| 9 | CPS1_HUMAN | Carbamoyl phosphate synthase 1 | 0.5 | 75/5.7 | 21 | 34 | 278 |
| 10 | CPS1_HUMAN | Carbamoyl phosphate synthase 1 | 0.5 | 75/5.8 | 26 | 45 | 378 |
| 11 | CPS1_HUMAN | Carbamoyl phosphate synthase 1 | 0.4 | 75/6 | 20 | 32 | 230 |
| 12 | CPS1_HUMAN | Carbamoyl phosphate synthase 1 | 0.4 | 75/6 | 15 | 23 | 213 |
| 13 | CYB5_HUMAN | Cytochrome b5 | 0.6 | 25/3.8 | 49 | 5 | 184 |
| 14 | ENPL_HUMAN | Endoplasmin precursor | 0.3 | 52/3.8 | 21 | 31 | 262 |
| 15 | ECHM_HUMAN | Enoyl CoA hydratase | 0.3 | 45/5.5 | 41 | 14 | 311 |
| 16 | GAMT_HUMAN | Guanidinoacetate N methyltransferase | 0.6 | 45/5.5 | 41 | 11 | 130 |
| 17 | K2C8_HUMAN | Keratin type II; cytoeskeletal 8 | 1.6 | 62/5 | 21 | 11 | 135 |
| 18 | K1C18_HUMAN | Keratina type I; cytoeskeletal 18 | 1.9 | 60/5 | 24 | 14 | 175 |
| 19 | RCN1_HUMAN | Reticulocalbin 1 precursor | 0.6 | 60/5 | 30 | 16 | 157 |
| 20 | NQO2_HUMAN | Ribosyldihydronicotinamide dehydrogenase | 2 | 45/4.8 | 25 | 7 | 188 |
| 21 | ALBU_HUMAN | Serum albumin precursor | 3.9 | 70/5.8 | 18 | 12 | 158 |
| 22 | ALBU_HUMAN | Serum albumin precursor | 2.1 | 70/6 | 45 | 33 | 366 |
| 23 | ALBU_HUMAN | Serum albumin precursor | 1.8 | 60/6.5 | 24 | 15 | 177 |
| 24 | ACDSB + ALBU_HUMAN | Short branched chain specific acyl CoA dehydrogenase + Serum albumin precursor | 0.6 | 57/5 | 28+24 | 12+14 | 135/126 |
| 25 | ACDSB HUMAN | Short branched chain specific acyl CoA dehydrogenase | 0.6 | 58/5.2 | 19 | 8 | 128 |
| 26 | ACDSB + ALDH2_ HUMAN | Short branched chain specific acyl CoA dehydrogenase+ aldheyde dehydrogenase | 0.6 | 57/5.2 | 17+11 | 7+7 | 138/112 |
| 27 | ACDSB_HUMAN | Short branched chain specific acyl CoA dehydrogenase | 0.6 | 57/5.2 | 51 | 27 | 387 |
| 28 | ACDSB_HUMAN | Short branched chain specific acyl CoA dehydrogenase | 0.6 | 57/5.8 | 29 | 13 | 189 |
| 29 | TERA_HUMAN | Transitional endoplasmin reticulum ATPase | 0.6 | 50/5 | 13 | 9 | 117 |
| 30 | TPM4_HUMAN | Tropomyosin alpha 4 chain | 1.6 | 45/3.8 | 64 | 26 | 311 |
| 31 | TBA1C_HUMAN | Tubulin alpha 1 chain | 0.6 | 55/5 | 18 | 7 | 115 |