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. 2011 Feb 1;10:27. doi: 10.1186/1476-511X-10-27

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Copyright ©2011 Karray et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Thin layer chromatography of PC and PE before or after incubation with ChPLA₂-IIA or ChPLA₂-IB. After solvent migration the silica plate was exposed to iodine vapor to reveal the various spots. Lane (1), PC. Lane (2), PC incubated with PLA₂from chicken pancreas (PLA₂-IB) and showing a complete hydrolysis of the PC. Lane (3), PC incubated with PLA₂from chicken intestine (PLA₂-IIA). Lane (4), PE. Lane (5), PE incubated with PLA₂-IB and showing a complete hydrolysis of the PE. Lane (6), PE incubated with PLA₂-IIA and showing a complete hydrolysis of the PE. Lane (7), free fatty acid C 18:1.