Table 1.
Flow sheet of chicken intestinal phospholipase A2 purification
| Purification Step | Total Activity (U)a | Total Protein (mg)b | Yield (%) | Specific activity (U/mg) | Purification Factor |
|---|---|---|---|---|---|
|
Extraction (pH 8.5) |
250 | 5520 | 100 | 0.045 | 1 |
| Acidic treatment (pH3) | 123 | 205.5 | 49 | 0.6 | 13.33 |
|
(NH4)2SO4 Precipitation (60%) |
108 | 108 | 43 | 1 | 22.22 |
| Sephadex G-50 | 95 | 6.41 | 38 | 14.8 | 328.88 |
| MonoS Sepharose | 25 | 0.156 | 10 | 160 | 3555 |
a) 1 Unit: μmole of fatty acid released per min using egg yolk as substrate in the presence of 10 mM NaTDC and in the presence of 10 mM CaCl2.
b) Proteins were estimated by Bradford method [30]. The experiments were conducted in triplicate.