Table 8.
Primer combinations for amplifying the complete mitochondrial sequences
| Primers | Nucleotide sequence (5'→3') | Primers | Nucleotide sequence (5'→3') |
|---|---|---|---|
| cytb-F1 | AATGACTTGAAGAACCACCGT | cytb-F2-2 | TTCTTTCCACCCATACTTT |
| cytb-R1 | CTCCGATCTTCGGATTACAAGAC | cytb-R2-2 | AGGAACCAGATGCCAGTA |
| CR-F | ACCCCTGGCTCCCAAAGC | 12SrRNA-F | ACAAAGCATAGCACTGAAGATG |
| CR-R | ATCTTAGCATCTTCAGTG | 12SrRNA-R | TTTGCATGGATGTCTTCTCG |
| 12SrRNA-R-R1 | GAAACAGTGCTTGAAGGAGG | 16SrRNA-F2 | CGCCTGTTTACCAAAAACATCG |
| 16SrRNA-F2-R1 | AGCCCTCGTTTAGCCATT | 16SrRNA-R2 | CCGGTCTGAACTCAGATCA |
| ND1-F | TCGACGAGGGGGTTTACGAC | ND2-2F | TCCTGGTGCTTCCTTTAC |
| ND1-R | AGTAGTTCCTAGTCCTAGGC | ND2-2R | AGCGGTTCCTACTATTCC |
| ND2-2R-F | GGCCTCGATCCTACAAAC | COI-F2 | TCCTCCTTCTCCTATCACT |
| COI-F2-F | CGTCCATTCCGACAGTAA | COI-R2 | CTGGGACTGCGTCTATTT |
| COII-F | AATTGCTCTACCATCCCT | ATPase-F | AAAGCGTTGGCCTTTTAAGC |
| COII-R | GCTCATTTATGTCCTCCT | CR-F-F | AAGGAGGACCCAAGAACG |
| CoNd3-2F | CTAAGCCTATACCTACAAGAA | ND4-2F | TTTCTTTACGCTTCTTCC |
| CoNd3-2R | AACATAAGAGTGCGGAGA | ND4-2R | GCAAATTGACCCTGTTAT |
| ND56-2F-2 | GCTACACTTATCCCAACC | ND56-2R-2-F | TGCCCTCTATGTAACCTGAT |
| ND56-2R-2 | TAACCCGATGTCTCCTAC | ND56-4F-F | TTCCTCGTGAGATGTCGT |
| ND56-4F | CTAGCCAACATGACGAGC | ||
| ND56-4R | TGAGAATGCGGTTGAAAT |