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. 2011 Feb 16;6(2):e14695. doi: 10.1371/journal.pone.0014695

Figure 6. Hsp70 is required for the association between CHIP and BAG5.

Figure 6

(A) PDAs with GST fusion proteins using lysates of H4 cells transfected with the CHIP deletion constructs CHIP ΔU and CHIP ΔTPR are shown. The blots were probed with anti-CHIP (upper) and anti-Hsp70 (lower) antibodies. The inputs shown are 10% of total lysates used in each assay. Molecular weight markers are indicated. Results are representative of three independent experiments. (B) PDAs were performed using GST fusion proteins and purified recombinant CHIP with or without Hsp70 as indicated. Proteins that associated with GST alone, GST-BAG5, GST-BAG5 DARA, or GST-BAG1 were probed with anti-CHIP (upper) and anti-Hsp70 (middle) antibodies. Input was 10% of proteins used for PDAs. The presence of equal amounts of GST fusion proteins was confirmed by Ponceau S staining (lower). Molecular weight markers are indicated. Results are representative of three independent experiments.