Figure 3. Subcutaneous (sc) injection of CRF₁/CRF₂ receptor antagonist, astressin-B induces pigmentation and hair growth in CRF-OE mice with alopecia.

Groups of 6–9 month old male or female CRF-OE mice were injected sc once daily for 5 consecutive days either with astressin-B (5 µg/mouse) or astressin₂-B (5 µg/mouse). A–C: A fully alopecic female CRF-OE mouse before (A) and at 1 (B) and 4 weeks (C, with 90% hair coverage), following the last sc astressin-B injection. D–F: A fully alopecic male mouse before (D) and at 1 (E) or 4 weeks (F) after the last sc injection of astressin₂-B, with no hair re-growth. G: Scores of skin pigmentation before the start of injection and 1 week after the last sc injection; H: time course of hair growth score before and up to 4 weeks after last sc injection. Data are individual and median in G and mean ± SEM in H. * p<0.05 vs week 0 (G and H); # p<0.05 vs astressin₂-B at the corresponding week (G) and (H).