Figure 1. Kinetics of antibody-induced pSn internalization.
(A) Confocal microscopical analysis of mAb 41D3-induced Sn internalization in primary porcine macrophages. Cells were incubated for the indicated times with mAb 41D3 at 37°C, fixed, permeabilized and stained with FITC-labelled goat-anti-mouse IgG. Images represent a single confocal z-section through the middle of the cell. (B) Kinetics of the percentage of macrophages with internalized Sn at different time points after incubation of macrophages at 37°C with mAb 41D3. Data represent the means ± standard deviations of 3 independent experiments. (C) Isotype matched control mAb 13D12 (gD of pseudorabies virus) shows no staining of primary macrophages. (D) Antibody internalization is not observed in primary macrophages stimulated with control mAb 74-22-15 (SWC3). (E) 41D3 F(ab')2 fragments lacking the Fc domain are internalized in primary macrophages. (F) Sn-specific mAb 41D3 induces internalization in CHO cells expressing recombinant pSn. Scale bar: 5 µm.