Figure 5. Impact on cyclin D, cyclin E and p27^Kip1 expression.

Mechanical ventilation for 24 h significantly decreased cyclin D1 and E1 mRNA (A) and protein (B and C) levels in lungs of 7-day old rats. In contrast, p27 ^kip1 mRNA increased (A). Inserts in (B) and (C) show cyclin D1 (B) and cyclin E1 (C) immunoblots of lung tissue of 7-day old rats ventilated for 24 h and non-ventilated 8-day old rats (controls). Blots were reprobed with β-actin for equal loading and transfer. Medians with 25^th and 75^th quartiles are shown, bars are 5^th and 95^th percentiles; qPCR, n = 6 rat pups per time group; immunoblot, n = 3 rat pups per time group. MV, mechanical ventilation. *p<0.05 versus non-ventilated group, ^§ p<0.05 versus 24 h ventilation.