Sequence alignment of PaNth
and other [4Fe-4S]-containing DNA glycosylase
homologs using the program ClustalW (68). The homologs are as follows (protein
code and GenBank accession number in parentheses): E.coli endonuclease
III (EcNth, P20625), M.luteus UV endonuclease (MlUVendo,
P46303), P.aerophilum endonuclease
III homolog (PaNth, AF222334), T.maritima MpgII
(TmMpgII, AAD35467), P.aerophilum T/G
and U/G mismatch-specific glycosylase (PaMig, AF222335)
and E.coli adenine DNA-glycosylase (EcMutY, P17802).
The conserved amino acid residues are shaded as follows: black,
strictly conserved identical amino acid; dark grey, conserved identical
amino acid residues; light grey, conserved similar amino acid residues.
The HhH motif is indicated. The cysteine residues for binding of [4Fe-4S] cluster
are marked with asterisks. The strictly conserved aspartic acid
residue is marked with a dot. The conserved lysine residue within
the endonuclease III family is marked with an arrowhead. The regions
A and B are indicated for sequence alignment in Figure 10.