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. 2001 Feb 1;29(3):604–613. doi: 10.1093/nar/29.3.604

Figure 2.

Figure 2

Expression and purification of PaNth protein. The 10% SDS–polyacrylamide gel contains the following samples: lysate of CSH100/pQE30 cells (lane 1), lysate of CSH100/pQE30PaNth after IPTG induction (lane 2), PaNth protein eluted from Ni2+-NTA column (lane 3), 70°C heat-treated PaNth (lane 4) and 90°C heat-treated PaNth (lane 5). The gel was visualized by Coomassie Blue staining. PaNth protein is indicated on the right. Lane M contains molecular mass standards (Bio-Rad) as indicated on the left.