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. 2001 Feb 1;29(3):604–613. doi: 10.1093/nar/29.3.604

Figure 3.

Figure 3

PaNth and EcNth glycosylase activity on DHT-containing substrates. The glycosylase reaction was carried out for 15 min with 1 nM *DHT/Y (Y = A, G, C or T) or *T/A, where the asterisk indicates the 5′-end-labeled strand. Each reaction mixture was then analyzed on a 15% denaturing polyacrylamide gel. The intact 96mer DNA (Intact) and cleaved 60mer product (Cleaved product) are indicated on the left. (A) Phosphoimage of the reaction in the presence (+) or absence (–) of 5 nM PaNth at 70°C. (B) Plot of the percentage cleaved versus PaNth concentration. Each individual point represented with error bars is as an average of data from two to five experiments. (C) Phosphoimage of the reaction with increasing amounts of EcNth at 37°C. (D) Plot of the percentage cleaved versus EcNth concentration.