Figure 5.

AP lyase activity of PaNth. The glycosylase reaction was carried out for 15 min. Each reaction mixture was then analyzed on a 15% denaturing polyacrylamide gel. The intact 96mer DNA (Intact) and cleaved 60mer product (Cleaved product) are indicated on the left. Asterisk indicates the 5′-end-labeled strand. (A) 1 nM *AP/G and increasing amounts of PaNth at 70°C. (B) 1 nM *DHT/G with: 0.4 nM PaNth (at 70°C), 10 nM EcNth (at 37°C) or 10 nM EcNth/5 nM Endo IV (at 37°C).