Figure 7.

PaNth activity on T/G and T/GO. The glycosylase reaction was carried out at 70°C for 15 min with (A) 1 nM *T/Y (Y = G, C, A and T) or (B) 1 nM *X/GO (X = G, C, A, T or U), where the asterisk indicates the 5′-end-labeled strand. Phosphoimages of the reactions on 15% polyacrylamide gels are shown with (+) or without (–) 50 nM PaNth. The intact 96mer DNA (Intact) and cleaved 60mer product (Cleaved product) are indicated on the left.