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. 2011 Feb 16;6(2):e16864. doi: 10.1371/journal.pone.0016864

Table 1. PCR primers for the amplification of Helicobacter pylori vacA and cagA regions.

Region	Primers	Sequence (5′ – 3′)	Product size	Source
*vacA* s	VA1F	ATGGAAATACAACAAACACAC	s1 176 bp	8
	VA1XR	CCTGARACCGTTCCTACAGC	s2 203 bp	29
*vacA* m	MF1	GTGGATGCYCATACRGCTWA^a	m1 107 bp	29
	MR1	RTGAGCTTGTTGATATTGAC^a	m2 182 bp	29
	y98vacAmF	CCTTGGAATTATTTTGACGC	m1 479 bp	30
	y98vacAmR	ATCCATGCGGTTATTGTTGT	m2 488 bp	30
	vacAmgapF	ATGCCAGCAAGAGCGATAAT	344 bp	This study
	vacAmgapR	GCATTGTGGCCTAGGGTTAG		This study
*cagA*	cagAF	TTGACCAACAACCACAAACCGAAG	183 bp	29
	cagAR	CTTCCCTTAATTGCGAGATTCC		29
	Ako982F	ACATTTTGGCTAAATAAACGCTG	360 bp	40
	Ako9825R	TCATGCGAGCGGCGATGTG		40
	cagAnegF	GAGAGGGTGGTGCGATAAAA	236 bp	This study
	cagAnegR	GGGCTATTTTATGGGGCATT		This study

^a

R is A or G, W is A or T, and Y is C or T.