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. 2011 Feb 16;6(2):e17037. doi: 10.1371/journal.pone.0017037

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Riemer et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Exon-intron structure of the human ERp90 gene. Vertical lines indicate positions of the 21 predicted exons. (B) Northern blot detection of the ERp90 mRNA in human tissue-culture cells. A nitrocellulose membrane with RNA from three different human tissue-culture cell lines was hybridized with a radioactively labeled ERp90 cDNA probe (top). The rRNA signals served as loading controls (bottom). (C) RT-PCR analysis of ERp90. Total RNA was isolated from various human tissue-culture cells, reverse transcribed and amplified with primers specific for ERp90 and actin. HeLa: cervical epithelial carcinoma; Huh7, HepG2: hepatocellular carcinoma; CF-PAC-1: pancreatic adeno carcinoma; A375, Meljuso: melanoma; HT1080: fibrosarcoma breast cancer; OVCAR3, SKOV3: ovarian epithelial carcinoma; LRB003, LRB010: embryonic stem cells.