Figure 6. HU challenge induces phosphorylation of Yox1 at its RXXST consensus.
(A) Cartoon diagram displaying the molecular arrangement of the homeodomain and the putative RXXS/T motif in Yox1p. Not to scale. (B) SDS-PAGE electrophoresis of Ha tagged Yox1 in untreated and HU-treated wt, Δcds1, and yox12A cells as described before. (C) RT-PCR analysis of cdc22+ transcript levels corresponding to fold induction over untreated wt for the same cells as in B and in rad3Δ cells before formaldehyde-induced cross-linking. Bars represent the average value, and error bars represent their SD, obtained by qPCR of triplicate biological samples. (D) Five-fold serially dilution volumes of wt, yox12A and cds1Δ, cells were spotted onto YES or YES plus indicated concentrations of HU or MMS.