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. 2011 Feb 16;6(2):e17138. doi: 10.1371/journal.pone.0017138

Figure 6. Simultaneous Inhibition of HDACs 1 and 6 Is Critical for Enhancing Cytarabine Induced-apoptosis in Pediatric AML Cells.

Figure 6

THP-1 cells were treated with HDACIs or cytarabine, all at Cmax concentrations, alone or combined for 3 h, washed three times with complete media and divided in two halves. One half of the cells was resuspended in drug-free complete media and cultured for up to 24 h. Effects of these treatments on apoptosis (panel A) and cell proliferation (as reflected in percent decrease of live cells relative to untreated cells, panel B) were determined by flow cytometry analysis as described in the Materials and Methods. ** indicates p<0.005. Whole cell lysates were extracted from the other half of the cells and subjected to Western blots probed by anti-p21, -c-Myc, -γH2AX, -Bim, or -β-actin antibody (panel C).