Figure 2. Different permeabilization conditions do not rescue the cytoplasmic staining of PADI6.

GV oocytes were prepared for IF with either 0.1% (A and B) or 5% (C and D) Triton X-100 concentration in the permeabilization solution instead of the normal 0.5% concentration. Primary antibody incubation was carried out at either 1 h RT (A and C) or O/N 4°C (B and D). PADI6 is shown in green and MSY2 is shown in red. Included are graphs of the averages and standard deviations of four intensity profiles of four images per condition of the different stains in either cortical (i and iv) or cytosolic (ii and iii) zones. Bars, 10 µm.