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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Nat Immunol. 2011 Jan 30;12(3):255–263. doi: 10.1038/ni.1993

Figure 4. Cytokine expression in eYFP+ and eYFP− CD4+ T cells in the draining LN and spinal cord.

Figure 4

(a) Lymph nodes from non-immune mice (day 0) as well as draining LN and spinal cord cells 6 and 15 days after EAE induction, respectively, were stained for CD4 and intracellular cytokines as indicated. The dot plots show cytokine expression profiles in gated eYFP+ CD4+ T cells (a) and gated eYFP− CD4+ T cells in (b). The data are representative for at least three independent experiments. (c) Cytokine concentrations measured in supernatant of 2×104 sorted eYFP+ or eYFP− CD4 T cells isolated from day 6 lymph nodes or day 15 spinal cord and restimulated in vitro with anti-CD3/anti-CD28 for 24h. Data show mean values +/− SD of cytokines measured from three individual mice.