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. Author manuscript; available in PMC: 2012 Jan 25.
Published in final edited form as: Curr Biol. 2011 Jan 13;21(2):167–172. doi: 10.1016/j.cub.2010.12.046

Figure 3.

Figure 3

MyoXVa enhances Eps8 accumulation and actin protrusion elongation activity in heterologous systems.

(A) A vestibular supporting cell expressing GFP-MyoXVa (green) shows enhanced labeling of Eps8 (red) in microvilli. Counterstained actin is shown in blue. Scale bar: 5 μm.

(B) In Cos-7 cells, the relative intensity (RI) of endogenous Eps8 labeling (red) at filopodia tips versus the cell cortex is significantly increased at filopodia tips in cells expressing GFP-MyoXVa (green) when compared to non-transfected or GFP-MyoX (green) transfected cells. Scale bar: 2 μm.

(C) Cos-7 cells overexpressing cherry-Eps8 (red) or MyoXVa (green) both show some elongation of filopodia, but when both are co-overexpressed, filopodia elongation is significantly enhanced. Scale bar: 2 μm. See also Figure S3.