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PN treatment reduces the amount of filaments formed as assessed by EM. The EM images depict negatively stained tau filaments that are representative of those used for quantitative EM measurements. PN-treated 5XY→F tau (E) formed less filaments compared to untreated wild-type tau (A). A dose-dependent reduction in filament formation was observed when PN-treated 5XY→F tau was added to untreated wild-type tau at ratios of 1:10 (B), 1:5 (C) and 1:1 (D). Scale bars are 0.5 µm.
