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. Author manuscript; available in PMC: 2012 Mar 10.
Published in final edited form as: Neuroscience. 2010 Dec 24;176:237–253. doi: 10.1016/j.neuroscience.2010.12.031

Table 1.

Rat Testing Protocol

Cohort Description Group N Treatment 30 Day DFP Exposure Period Procedure/Testing Days 14 Day Drug-Free Washout Period Procedure/Testing Days
Behavioral Cohort Grip Strength Open Field Rotarod Grip Strength Open Field Rotarod NOR WM PPI
A 26 VEH 7,14,21,28 8,22 16,30 35,42 37 34,44 33-37 38-44 41-43
B 26 DFP 0.25 7,14,21,28 8,22 16,30 35,42 37 34,44 33-37 38-44 41-43
C 15 DFP 0.5 7,14,21,28 8,22 16,30 35,42 37 34,44 33-37 38-44 41-43
D 19 DFP 0.75 7,14,21,28 8,22 16,30 35,42 37 34,44 33-37 38-44 41-43
E 11 DFP 1.0 7,14,21,28 8,22 16,30 35,42 37 34,44 33-37 38-44 41-43
Blood Draw Blood Draw
Plasma Cohort A 4 VEH 0,7,14,21,30 37, 44
B 4 DFP 0.25 0,7,14,21,30 37, 44
C 4 DFP 0.5 0,7,14,21,30 37, 44
D 4 DFP 0.75 0,7,14,21,30 37, 44
E 4 DFP 1.0 0,7,14,21,30 37, 44
Sacrifice
Brain AChE & ChAT Cohort 1 A 4 VEH 30
B 4 DFP 0.25 30
C 4 DFP 0.5 30
D 4 DFP 0.75 30
E 4 DFP 1.0 30
Sacrifice
Brain AChE & ChAT Cohort 2 A 4 VEH 37
B 4 DFP 0.25 37
C 4 DFP 0.5 37
D 4 DFP 0.75 37
E 4 DFP 1.0 37
Brain AChE & ChAT Cohort 3 A 4 VEH Sacrifice
B 4 DFP 0.25 44
C 4 DFP 0.5 44
D 4 DFP 0.75 44
E 4 DFP 1.0 44

VEH, vehicle; DFP, diisopropylfluorophosphate; NOR, novel object recognition, WM, water maze, PPI, prepulse inhibition; ChAT, choline acetyltransferase, AChE, acetylcholinesterase