Table 1.

Primers used for real-time Pcr

	Occludin	Claudin-5	Collagen-IV	MMP-2	MMP-9	GAPDH
F	5′-ATGATGAACAG CCCCCTAATGT-3′	5′-TGTGAGGACT TGACCGACCTT-3′	5′-GAGGGTGCTG GACAAGCTCTT-3′	5′-GGTGGTGGTCA CAGCTATTTCTT-3′	5′-GGATCCCCC AACCTTTACCA-3′	5′-TGAACGGGA AGCTCACTGG-3′
R	5′-TGTCGACTCTT TCCGCATAGTC-3′	5′-CGGCAGTTTG GTGGCTACTT-3′	5′-TAAACGGACTG GGCTCGGAATTC-3′	5′-GCCAGTCCGAT TTGATGCTT-3′	5′-AAGGTCAGAAC CGACCCTACAA-3′	5′-TCCACCACCC TGTTGCTGTA-3′

Data were normalized against GAPDH expression and were expressed as fold-differences between control and treated groups.

MMP, metalloproteinase.