Skip to main content
. 2009 Mar;31(3):123–131. doi: 10.1111/j.1365-3024.2008.01088.x

Figure 1.

Figure 1

Bone marrow macrophages (BMMΦ) stimulated with parasite specific E/S show no consistent change in co-stimulatory cell surface markers compared to unstimulated levels. Bone marrow was isolated and pooled from 3–5 naïve C57BL/6 mice and cultured into BMMΦ. The BMMΦ were incubated for 48 h, in vitro with medium alone – unstimulated (U), 50 µg/mL E isolate E/S (E), 50 µg/mL J isolate E/S (J), 50 µg/mL S isolate E/S (S) or 100 ng Lipopolysaccharide (LPS). The cells were analysed for cell-specific markers F4/80, CD11b, co-stimulatory markers CD80 and CD86 and antigen recognition marker MHCII. A representative scatter plot is shown (a) with analysis performed on cells negative for 7AAD-R1 (b) and double positive for CD11b and F4/80 (c). Levels of CD80, CD86 and MHC class II were then measured on these cells with a representative histogram showing CD80 expression on untreated cells (filled histogram) compared to its relevant isotype control (unfilled histogram) (d). Geomeans for unstimulated, E, J or S isolate antigen and LPS stimulated BMMΦ and relevant isotype controls were calculated and a fold change for Geomean anti cell surface marker antibody/Geomean Isotype control antibody was plotted for CD80, CD86 and MHCII expression on F480+CD11b+7AAD−cells (e). Experiments were performed four times and data shown are a representative experiment.