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. 2001 Feb 1;29(3):767–773. doi: 10.1093/nar/29.3.767

Figure 1.

Figure 1

Inhibition of RNA polymerase II transcription by juglone in vitro. (A) Pulse–chase transcription assay using a linearized CMV template, HeLa nuclear extract with recombinant P-TEFb added to enhance productive elongation. Juglone was added during preincubation at the indicated concentrations. Run-off transcripts generated were analyzed in a 6% denaturing gel. (B) Quantitation of the run-off transcripts. The IC50 for the inhibitory effect of juglone on RNA polymerase II transcription was determined as described in Materials and Methods.