Skip to main content
. 2001 Feb 1;29(3):767–773. doi: 10.1093/nar/29.3.767

Figure 2.

Figure 2

RNA polymerase II transcription is not affected by Pin1 in vitro or in vivo. (A) SDS–PAGE of the indicated three purified recombinant Pin1 proteins. M, 10 kDa ladder as a marker; lane 1, Pin1 (2 µl); lane 2, Pin1H59A (5 µl); lane 3, Pin1WW (0.4 µl). (B) Pulse–chase transcription assay with the indicated amounts (1 or 4 µl) of Pin1 proteins. Labeled transcripts were analyzed on 6% denaturing gel. (C) Co-transfection assay with a reporter (CMV-luc) and a Pin1 expression construct (pCDNA3-HA-Pin1 or pCNDA3-HA-Pin1H59A). The amounts of Pin1 constructs used are indicated.