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. 2001 Feb 1;29(3):767–773. doi: 10.1093/nar/29.3.767

Figure 3.

Figure 3

Specific disruption of the RNA polymerase II preinitiation complexes by juglone. (A) Juglone (50 µM) was added at different stages of a pulse–chase reaction as indicated: none (no drug added), preinc. (preincubation), pulse (chase). Reactions were stopped after the pulse (P) or after the chase (C). (B) Identical experiment with NEM (1 mM) instead of juglone. (C) Juglone (5× 35 µM; 15× 105 µM) and NEM (5× 580 µM; 15× 1740 µM) were added after 15–20 min preincubation and incubated for an additional 3 or 10 min. The reactions were stopped after the pulse. Labeled transcripts were analyzed on 6 (A and B) or 9% (C) denaturing gels.