Table 1.
Overview of nuclear diffusion coefficients measured by SMT and FCS
| Reference | Construct | Method | Diffusion coefficient | Subcompartment |
|---|---|---|---|---|
| (Grunwald et al. 2006b) | U1 snRNP bioactive | SMT | D = 0.5 to 8 μm2/sa | Nucleoplasm, speckles |
| (Grunwald et al. 2008a) | Streptavidin-NLS inert | SMT | D = 0.8 to 5 μm2/sa | Nucleoplasm, nucleolus, heterochromatin |
| (Speil and Kubitscheck 2010) | Ovalbumin inert | SMT | D = 0.5 to 12 μm2/sa | Nucleoplasm, nucleolus |
| (Bancaud et al. 2009) | GFP-monomer | FCS | D1 = 29 μm2/s | Euchromatin |
| GFP-monomer | D1 = 87 μm2/s | Aqueous solution | ||
| GFP-dimer | D2 = 17 μm2/s | Euchromatin | ||
| GFP-dimer | D2 = 55 μm2/s | Aqueous solution | ||
| GFP-pentamer | D3 = 7.7 μm2/s | Nucleoplasm | ||
| (Politz et al. 1998) | Oligo(dT):poly(A) RNA | FCS | D = 1 to 10 μm2/s | Nucleoplasm |
| (Politz et al. 2006) | Oligo(dT):poly(A) RNA | FCS | D1 = 0.65 | Speckles |
| D2 = 0.7 | Nucleoplasm | |||
| (Shav-Tal et al. 2004) | YFP-MS2 mRNP | FRAP | D = 0.09 μm2/s | Nucleoplasm |
| SMT | D = 0.04 μm2/s | Nucleoplasm | ||
| (van den Bogaard and Tyagi 2009) | GFP-mRNA-96-mer | SMT | D = 0.033 μm2/s | Nucleus |
| (Siebrasse et al. 2008) | DNA-labeled BR mRNP | SMT | D = 0.24 to 4.0 μm2/s | Salivary-gland cell nucleus |
| (Mor et al. 2010) | Different sized Dys mRNA | SMT | D = 0.005 μm2/s | Nucleus |
| (Maertens et al. 2005) | HIV-IN | FCS | D = 10.5 μm2/s | Nucleus |
aIn these studies, diffusion coefficients were fixed to compare the relative amounts of the different mobility classes in different compartments. For details, see original literature