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RNase H mapping of gCYb-558 and 5′CYbPES1T crosslink. Lanes 1–4, 5′CYbPES1T mRNA only; lanes 5–8, 3′-crosslinked gCYb-558 and 5′CYbPES1T. All lanes were treated with RNase H. Thick horizontal lines on the left, mRNA alone; thick and thin lines on the right, crosslinked mRNA and gRNA, respectively.
