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Accurate cleavage of 3′-crosslinked 5′CYbUT and gCYb-558. Crosslinks (mRNA 3′-end-labeled) were assayed for gRNA-directed cleavage using standard cleavage conditions. Lane 1, T1 digest of 5′CYbUT; lanes 2–4, 3′-crosslinked RNAs: lane 2, without mitochondrial fraction; lane 3, with mitochondrial fraction; lane 4, with mitochondrial fraction and a 10-fold excess of free gCYb-558. X-link, 3′-crosslinks; mRNA, free 5′CYbUT from broken crosslinks; arrow, cleavage products.
