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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 Nov 1;185(11):6555–6562. doi: 10.4049/jimmunol.1000135

Figure 6. Role of TRAF3 in secretion of IL-6 and IgM following hCD40-P227A or hCD40Wt signaling.

Figure 6

A. CH12.LX cells stably expressing matched amounts of both mCD40 and hCD40-Wt (hCD40Wt.T3+/+ or hCD40Wt.T3−/−), or hCD40-P227A (P227A.T3+/+ or P227A.T3−/−) were stimulated with medium only (BCM) or 10 µg/ml agonistic anti-mCD40, anti-hCD40, or isotype control mAbs (m-iso, h-iso) for 48 hours. Culture supernatants were analyzed by quantitative IL-6 ELISA. Data represent means ± s.d. of triplicate cultures, and are representative of three independent experiments. At least two separate subclones of each cell line were tested with similar results (not shown). *=p<0.05; **=p<0.001 by Student’s t-test.

B. CH12.LX cells stably expressing matched amounts of both mCD40 and hCD40-Wt hCD40-Wt (hCD40Wt.T3+/+ or hCD40Wt.T3−/−), or hCD40-P227A (P227A.T3+/+ or P227A.T3−/−) were stimulated with 2 µg/ml agonistic anti-mCD40, anti-hCD40, or isotype control mAbs for 72 hours. IgM-secreting cells in replicate cultures are enumerated (±SE) as described (14). Results are representative of 3 similar experiments. Two separate subclones of each cell line were tested with similar results (not shown). * = p<0.05; ** = p<0.001 by Student’s t-test.