Table 4.

Differences Between Fitted and Actual Values of EC50, Slope, y_min, and y_max by XLfit without Prefit

Parameter	No1	No2	No3	No4	Median Dif	Max Dif	pvalue	r
EC50	10	0	0	8	0	3.58	<2.2e-16	0.775
	10	1	0	22	0.13	37.17	0.2758	0.11
	10	2	0	39	1.41	10.58	0.02382	0.226
	10	3	0	49	1.93	10.4	0.01612	0.24
	10	4	0	40	1.56	24.79	0.009522	0.258
	20	0	0	6	0	3.61	<2.2e-16	0.929
	20	2	0	14	0.09	11.73	<2.2e-16	0.709
	20	4	0	11	0.19	8.3	1.23E-12	0.635
	20	6	0	13	0.22	10.42	<2.2e-16	0.73
	20	8	0	19	0.34	12.78	4.53E-08	0.514
slope	10	0	0	32	0	4.82	8.28E-07	0.47
	10	1	0	46	1.13	6.84E+15	0.0933	-0.169
	10	2	0	75	2.3	189.41	0.9782	0.003
	10	3	0	78	2.02	120.51	0.4397	0.078
	10	4	0	76	3.21	253.95	0.878	0.016
	20	0	0	15	0	4.48	<2.2e-16	0.746
	20	2	0	41	0.89	66.5	0.9688	0.004
	20	4	0	55	1.34	56.63	0.4695	0.073
	20	6	0	57	1.56	135.52	0.02047	0.232
	20	8	0	67	1.9	104.98	0.4246	0.081
ymin	10	0	0	3	0	1.79E+03	0.06308	0.187
	10	1	0	13	5.73	2.75E+10	0.3146	0.102
	10	2	0	5	13.25	2.77E+03	0.4586	0.075
	10	3	0	5	13.46	4.79E+04	0.8188	0.023
	10	4	0	9	14.21	1.99E+03	0.7425	0.033
	20	0	0	4	0	976	0.534	0.063
	20	2	0	3	2.96	762.23	0.3416	0.096
	20	4	0	1	3.85	358.8	0.01858	0.235
	20	6	0	4	4.79	7.93E+03	0.325	0.099
	20	8	0	5	6.42	6.71E+03	0.6725	0.043
ymax	10	0	0	3	0	2.23E+03	0.1889	-0.132
	10	1	0	1	5.49	1.86E+03	0.01008	0.256
	10	2	0	19	48.94	1.01E+06	0.1667	-0.139
	10	3	0	30	75.75	7.36E+07	0.895	0.013
	10	4	0	30	75.74	1.69E+08	0.5535	0.06
	20	0	0	0	0	99.51	<2.2e-16	0.81
	20	2	0	7	3.82	3.12E+04	0.6598	-0.045
	20	4	0	9	6.57	4.59E+07	0.6877	-0.041
	20	6	0	8	6.12	1.66E+07	0.4453	0.077
	20	8	0	5	7.02	2.77E+04	0.3871	-0.087

No1: number of data points; No2: number of randomly perturbed data points; No3: number of curves without fit; No4: number of curves with absolute differences in EC50, y_min and y_max > 2.0, 200.0% and 200.0%, respectively and maximum difference in ratio of slope > 2.0 or < 0.5; Median Dif: median difference; Max Dif: maximum difference; pvalue: significance of Pearson correlations; and r: Pearson correlation coefficient.