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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Oncogene. 2009 Dec 21;29(11):1691–1701. doi: 10.1038/onc.2009.458

Figure 2.

Figure 2

Over-expression of Cyp40, FKBP51 PCa cell lines. (a) WCEs from normal prostate epithelial cells (PrEC), androgen-dependent (AD) (LNCaP, LAPC-4, VCaP and DuCaP) and- independent (AI) (C4-2, C-81 and CWR22R) human PCa cell lines were prepared and analyzed by Western blot for expression of the AR, FKBP51, Cyp40 and actin, respectively, as described in Materials and Methods. (b) Densitometric scanning of the bands corresponding to protein expression of Cyp40, FKBP51 and the AR in PrEC, AD and AI human prostate cell lines. Cyp40, FKBP51 and AR protein levels were expressed as relative expression levels, taking the values obtained from LNCaP cells as 1. Results are representative of two independent experiments. AD vs PrEC, **P values ranged from <0.01 to <.001. AI vs AD, ##P<0.01.