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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Oncogene. 2009 Dec 21;29(11):1691–1701. doi: 10.1038/onc.2009.458

Figure 4.

Figure 4

Down-regulation of FKBP51 inhibits AR activity. LNCaP cells were transiently transfected with FKBP51 siRNA or non-target control siRNA. Ninety-six hours later, the WCEs were prepared, and expression of FKBP51, FKBP52 and actin (control) was measured by Western blotting (a). LNCaP cells were transiently transfected with FKBP51 siRNA or non-target control siRNA, and the PSA-Luc promoter reporter construct along with a β-gal plasmid. Forty-eight hours later, cells were treated with or without R1881, FK506, FK506 and R1881 for 24 h. Lysates were prepared and analyzed for the AR and FKBP51 expression by Western blotting (b) and for AR reporter gene activity (c). Results are means ± SEM of two independent experiments. *P<0.05 and **P<0.01 vs R1881 control siRNA.