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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Med Sci Sports Exerc. 2009 Aug;41(8):1597–1605. doi: 10.1249/MSS.0b013e31819f1f05

Figure 1.

Figure 1

Southern blot hybridization analysis of p53+/− Wnt-1 TG mammary tumor DNA demonstrates loss of the wildtype p53 allele in a subset of tumors. Genomic DNA was prepared from p53+/− Wnt-1 TG mammary tumors and restricted with Bam HI prior to agarose gel electrophoresis and Southern blot hybridization with a labeled mouse p53 cDNA probe (from p53 exons 2–6)(13). Fourteen representative tumor DNAs are shown. Six of these 14 tumors show definitive loss of the wildtype p53 allele (LOH) (fastest migrating band). Residual presence of a wild type (WT) allele in these LOH tumors is ascribed to stromal and normal cell contamination of the tumor samples.