Figure 2.

Location of the S7 mutations in the S7 structure. Crystallographic structure of S7 adapted from Hosaka et al. (1), showing the deletion and point mutations that were investigated in this study. In the case of the β-hairpin deletion, the 72–89 sequence was replaced with a short flexible loop, RRGGGGS, so as not to perturb the structure of the protein (5). The regions of S7 involved in binding to 16S rRNA, as determined by mutagenesis studies (4,5) and analysis of the crystal structures of the 30S subunit (17,18), are indicated by arrows.