Figure 7.

Measurement of the BPS immunofluorescence stains after 14 days of culture. Five arbitrarily selected areas were counted for each sample. Compared to the blank control, the presence of glass granules (0%, 2%, and 5% Zn) significantly enhanced the production of BSP, and this effect was greater in the presence of larger quantity of granules (10 < 30). Data were analyzed by student t-test for n = 5 (*P < .05: comparison with respect to blank, ^#P < .05: comparison between glass quantity 10 versus 30, and ^●P < .05: comparison between zinc-free and zinc-added glass).