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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: J Biol Chem. 2004 Aug 31;279(46):48177–48188. doi: 10.1074/jbc.M407836200

Fig. 4. Specific uracil-DNA glycosylase activity of R276X mutant proteins.

Fig. 4

Uracil-DNA glycosylase activity was measured under standard reaction conditions using 0.04–0.13 units of UNG*, UNG, or the indicated R276X mutant protein, as described under “Experimental Procedures.” Protein concentrations were determined using the Bradford method (36) and the Protein Assay reagent (Bio-Rad). The specific activity (units/mg) of UNG* and the R276X mutant enzymes was normalized to that of UNG (3.76 × 105 units/mg), which was defined as 100%. Arg276 mutant proteins are denoted by single letter amino acid abbreviations. Error bars represent the standard deviation of four experimental determinations.