Figure 3. Temperature-compensation of circadian peroxiredoxin oxidation rhythms.
Red blood cells were entrained in temperature cycles (12 h at 32°C, 12 h at 37°C) for two complete cycles and then kept under a constant temperature of either 32°C or 37°C for the rest of the experiment and sampled every 4 hours as before. Immunoblots for oxidised/hyperoxidised peroxiredoxin (PRX-SO2/3) dimer obtained from red blood cells from subjects A, B and C are shown. Loading controls (Coomassie-stained gels showing haemoglobin monomer bands) for each blot are also shown. Quantification of the above immunoblots by densitometry is shown on the left of the figure.
