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. 2001 Feb 1;29(3):716–724. doi: 10.1093/nar/29.3.716

Figure 1.

Figure 1

Schematic representation pΔMSP1#2. This plasmid includes a 1.2 kb XhoI (X) insert that contains MSP-1 targeting sequence (MSP-1 KO). The first 900 bp of this insert are P.falciparum MSP-1 sequence and this region was used as a probe throughout these studies (MSP-1 probe). The location of the selectable marker cassette (TgDHFR cassette), the TgDHFR-TS probe and the HSP86 3′-UTR (HSP3′) are shown. A representation of the P.falciparum MSP-1 gene is shown below the plasmid. The locations of relevant restriction enzyme XmnI (Xm), XbaI (Xb), BglII (B), SpeI (S) and EcoRI (E) are indicated. All sizes are to scale with the exception of the plasmid backbone (dashed line).