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. 2001 Feb 1;29(3):716–724. doi: 10.1093/nar/29.3.716

Figure 2.

Figure 2

Analysis of transfected D10-ΔMSP1#2 parasite populations at different time points. (A) PFGE separating chromosomes from parental D10 (lane 1) and transfected parasite populations of D10-ΔMSP1#2 after no (lane 2) or one to three cycles in culture (lanes 3–5). Following transfer to a nitrocellulose membrane, the filter was hybridised with the 900 bp MSP-1 probe. Chromosome 9, the location of the endogenous MSP-1 gene, is indicated (chr9). (B) PFGE separating chromosomes from parental D10 (lane 1) and transfected parasite populations of D10-ΔMSP1#2 after no and two drug cycles either on drug selection (lanes 2 and 4, respectively) or maintained in the absence of pyrimethamine selection for 5 weeks (lanes 3 and 5, respectively). Southern blotting and hybridisation was as described in (A).