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. 2001 Feb 1;29(3):716–724. doi: 10.1093/nar/29.3.716

Figure 6.

Figure 6

Recovery in E.coli and retransfection in P.falciparum of SRF plasmids. (A) Ethidium bromide-stained agarose gel showing plasmid DNA prepared from individual E.coli colonies obtained following transformation with DpnI-treated SRF gDNA and digested with a combination of BamHI and EcoRI. The pΔMSP1#2 parent plasmid is included (parent). DNA markers in kb (M) are indicated to the left. (B) PFGE of recSRF chromosomes obtained from retransfection of an E.coli recovered SFR plasmid pool. Lane 1, parental D10; lane 2, URF; lane 3, recSRF; lane 4, SRF. (C) recSRF chromosomes were digested at 25°C overnight with varying units of SmaI: lane 1, 0 U; lane 2, 0.01 U; lane 3, 0.1 U; lane 4, 1 U; lane 5, 2 U; lane 6, 3 U. The DNA was transferred to a nitrocellulose membrane and hybridised with the MSP-1 probe.