Figure 7.

Plasmid backbone is sufficient for plasmid replication in parasites. An RsaI plasmid library was constructed from P.falciparum gDNA and introduced into both E.coli (left) and P.falciparum (right). In the case of P.falciparum, transfected parasites were cultured for 4 days before preparation of DpnI-treated gDNA and recovery in E.coli. DNA was extracted from 18 random colonies in each case and digested with a combination of EcoRI and PstI. The restricted plasmids were electrophoresed on 1% agarose gels containing ethidium bromide and visualised via UV transilluminator. DNA markers in kb (M) are indicated to the left.