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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: RNA Biol. 2010 May–Jun;7(3):373–380. doi: 10.4161/rna.7.3.11693

Figure 8.

Figure 8

Dual luciferase reporter assays were used to validate miR-107 target sites on INSIG1 mRNA. A sequence in the INSIG1 mRNA open reading frame (nts 370–518) that contains two TGCTGC iterations (top) was subcloned into pRL-TK reporter plasmid (INSIGMRE). The pRL-TK plasmid containing the mutated sequence of INSIGMRE (INSIGMREmut, which is only subtly different as shown) was constructed in parallel. Plasmid transfection, miRNA transfection, and dual luciferase activity assay were followed our published protocols (See Methods section). The figure showed that INSIGMRE specifically responds to miR-107 transfection but not to miR-320, or to a negative control miRNA. “*”-Student’s one-tailed t-test p < 0.001.