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Sequence analysis in maternal plasma samples. The lowest (1) and the highest (2) enrichment of fetal mutated allele are shown following COLD-PCR-sequencing; results on fetal DNA extracted from chorionic villi are depicted in (3); the fetal mutated allele was not detectable using conventional PCR-sequencing (4); no mutated peak is visible when the fetus inherited the paternal wild-type allele (5).
