Skip to main content
. 2011 Feb 17;7(2):e1001291. doi: 10.1371/journal.ppat.1001291

Figure 1. Caspase 3 activation is blocked in RV infected cells.

Figure 1

A. A549 were infected with RV (MOI = 1) for 42 hours after which they were treated with staurosporine (ST) for 6 hours. Samples were then processed for indirect immunofluorescence using rabbit anti-caspase 3 and mouse anti-capsid. Primary antibodies were detected with donkey anti-rabbit Alexa488 and chicken anti-mouse Alexa594. Nuclei were counter stained with DAPI. Scale bar  = 20 µm. B. The percentages of cells among mock and RV infected cells treated with ST expressing active caspase 3 were determined and plotted. Student's t-Test was performed to determine statistical significance. p = ≤0.001. Percentages were determined from three independent experiments in which at least 100 cells for each experiment were scored. C. To demonstrate that ST treatment did not result in selective loss of infected cells, the percentages of RV infected cells were determined in control (RV) and ST-treated (RV + ST) samples. D. The percentage of viable cells in mock and RV infected samples were determined by Trypan blue dye exclusion at the indicated time points. Error bars indicate standard deviations calculated from three independent experiments.