Figure 4. RV infection activates Bax.

A. A549 cells were infected with RV (MOI = 1) and then processed for indirect immunofluorescence after 48 hours. Samples were stained with goat anti-RV, rabbit anti-cytochrome c and a mouse monoclonal (6A7) that recognizes activated Bax. Primary antibodies were detected with chicken anti-goat Alexa488, donkey anti-rabbit Alexa637, and chicken anti-mouse Alexa594. B. A549 cells were transiently transfected with plasmids encoding RV capsid, glycoproteins E2 and E1 or vector alone. After 48 hours, samples were processed for indirect immunofluorescence as described in A. Images shown are representative of three independent experiments in which at least 100 infected or transfected cells were examined. Scale bar  = 10 µm.