Figure 6. Capsid expression protects against Bax- but not Bak-induced apoptosis.

A549 cells were co-transfected with plasmids encoding GFP-Bax or GFP-Bak together with plasmids encoding capsid, CapNT, Bcl-XL (not shown) or vector alone. After 24 hours, samples were stained with TMRM for 30 minutes and then subjected to flow cytometric analyses. Sample FACS plots for GFP-Bax (A) and GFP-Bak (B) transfectants are shown. C. The levels of relative specific cell death in GFP positive cells were calculated and plotted. Error bars indicate standard deviations calculated from three independent experiments. *p≤0.001, **p≤0.01.