Figure 13. Vero cells that were infected with the CR5A mutant or wild type RV were treated with DMSO (-) or Z-VAD-FMK (50 µM) every day for up to 3 days.

A. Cell lysates and culture supernatants were harvested at indicated time periods. Equal amounts of cell lysates (60 µg) were subjected to immunoblot analyses with antibodies to viral (p150 and capsid) and cellular proteins (GAPDH). At each time point, the relative signals of the p150/GAPDH and capsid/GAPDH in DMSO-treated cells were normalized to 1.0. Relative ratios of Z-VAD-FMK to DMSO samples were then determined. In some cases (ND), it was not possible to determine the relative ratios due to low signal intensity. B. Media from infected cells were serially diluted 10-fold before addition to RK-13 monolayers for plaque assays. Based on the clearing of cell monolayers, it can be seen that supernatants from CR5A infected Z-VAD-FMK-treated Vero cells contain more infectious virus.